Growing Doubt: a Scientist’s Experience of GMOs

August 31, 2015

http://www.independentsciencenews.org/health/growing-doubt-a-scientists-experience-of-gmos/

Jonathan R. Latham, PhD
By training, I am a plant biologist. In the early 1990s I was busy making genetically modified plants (often called GMOs for Genetically Modified Organisms) as part of the research that led to my PhD. Into these plants we were putting DNA from various foreign organisms, such as viruses and bacteria.
I was not, at the outset, concerned about the possible effects of GM plants on human health or the environment. One reason for this lack of concern was that I was still a very young scientist, feeling my way in the complex world of biology and of scientific research. Another reason was that we hardly imagined that GMOs like ours would be grown or eaten. So far as I was concerned, all GMOs were for research purposes only.
Gradually, however, it became clear that certain companies thought differently. Some of my older colleagues shared their skepticism with me that commercial interests were running far ahead of scientific knowledge. I listened carefully and I didn’t disagree. Today, over twenty years later, GMO crops, especially soybeans, corn, papaya, canola and cotton, are commercially grown in numerous parts of the world.

Jonathan Latham

Depending on which country you live in, GMOs may be unlabeled and therefore unknowingly abundant in your diet. Processed foods (e.g. chips, breakfast cereals, sodas) are likely to contain ingredients from GMO crops, because they are often made from corn or soy. Most agricultural crops, however, are still non-GMO, including rice, wheat, barley, oats, tomatoes, grapes and beans.
For meat eaters the nature of GMO consumption is different. There are no GMO animals used in farming (although GM salmon has been pending FDA approval since 1993); however, animal feed, especially in factory farms or for fish farming, is likely to be GMO corn and GMO soybeans. In which case the labeling issue, and potential for impacts on your health, are complicated.
I now believe, as a much more experienced scientist, that GMO crops still run far ahead of our understanding of their risks. In broad outline, the reasons for this belief are quite simple. I have become much more appreciative of the complexity of biological organisms and their capacity for benefits and harms. As a scientist I have become much more humble about the capacity of science to do more than scratch the surface in its understanding of the deep complexity and diversity of the natural world. To paraphrase a cliché, I more and more appreciate that as scientists we understand less and less.

The Flawed Processes of GMO Risk Assessment

Some of my concerns with GMOs are “just” practical ones. I have read numerous GMO risk assessment applications. These are the documents that governments rely on to ‘prove’ their safety. Though these documents are quite long and quite complex, their length is misleading in that they primarily ask (and answer) trivial questions. Furthermore, the experiments described within them are often very inadequate and sloppily executed. Scientific controls are often missing, procedures and reagents are badly described, and the results are often ambiguous or uninterpretable. I do not believe that this ambiguity and apparent incompetence is accidental. It is common, for example, for multinational corporations, whose labs have the latest equipment, to use outdated methodologies. When the results show what the applicants want, nothing is said. But when the results are inconvenient, and raise red flags, they blame the limitations of the antiquated method. This bulletproof logic, in which applicants claim safety no matter what the data shows, or how badly the experiment was performed, is routine in formal GMO risk assessment.
To any honest observer, reading these applications is bound to raise profound and disturbing questions: about the trustworthiness of the applicants and equally of the regulators. They are impossible to reconcile with a functional regulatory system capable of protecting the public.

The Dangers of GMOs

Aside from grave doubts about the quality and integrity of risk assessments, I also have specific science-based concerns over GMOs. I emphasise the ones below because they are important but are not on the lists that GMO critics often make.
Many GMO plants are engineered to contain their own insecticides. These GMOs, which include maize, cotton and soybeans, are called Bt plants. Bt plants get their name because they incorporate a transgene that makes a protein-based toxin (usually called the Cry toxin) from the bacterium Bacillus thuringiensis. Many Bt crops are “stacked,” meaning they contain a multiplicity of these Cry toxins. Their makers believe each of these Bt toxins is insect-specific and safe. However, there are multiple reasons to doubt both safety and specificity. One concern is that Bacillus thuringiensis is all but indistinguishable from the well known anthrax bacterium (Bacillus anthracis) (1). Another reason is that Bt insecticides share structural similarities with ricin. Ricin is a famously dangerous plant toxin, a tiny amount of which was used to assassinate the Bulgarian writer and defector Georgi Markov in 1978. A third reason for concern is that the mode of action of Bt proteins is not understood (Vachon et al 2012); yet, it is axiomatic in science that effective risk assessment requires a clear understanding of the mechanism of action of any GMO transgene. This is so that appropriate experiments can be devised to affirm or refute safety. These red flags are doubly troubling because some Cry proteins are known to be toxic towards isolated human cells (Mizuki et al., 1999). Yet we put them in our food crops.
A second concern follows from GMOs being often resistant to herbicides. This resistance is an invitation to farmers to spray large quantities of herbicides, and many do. As research recently showed, commercial soybeans routinely contain quantities of the herbicide Roundup (glyphosate) that its maker, Monsanto, once described as “extreme” (Bøhn et al 2014).
Glyphosate has been in the news recently because the World Health Organisation no longer considers it a relatively harmless chemical, but there are other herbicides applied to GMOs which are easily of equal concern. The herbicide Glufosinate (phosphinothricin, made by Bayer) kills plants because it inhibits the important plant enzyme glutamine synthetase. This enzyme is ubiquitous, however, it is found also in fungi, bacteria and animals. Consequently, Glufosinate is toxic to most organisms. Glufosinate is also a neurotoxin of mammals that doesn’t easily break down in the environment (Lantz et al. 2014). Glufosinate is thus a “herbicide” in name only.
Thus, even in conventional agriculture, the use of glufosinate is hazardous; but With GMO plants the situation is worse yet. With GMOs, glufosinate is sprayed on to the crop but its degradation in the plant is blocked by the transgene, which chemically modifies it slightly. This is why the GMO plant is resistant to it; but the other consequence is that when you eat Bayers’ Glufosinate-resistant GMO maize or canola, even weeks or months later, glufosinate, though slightly modified, is probably still there (Droge et al., 1992). Nevertheless, though the health hazard of glufosinate is much greater with GMOs, the implications of this science have been ignored in GMO risk assessments of Glufosinate-tolerant GMO crops.
A yet further reason to be concerned about GMOs is that most of them contain a viral sequence called the cauliflower mosaic virus (CaMV) promoter (or they contain the similar figwort mosaic virus (FMV) promoter). Two years ago, the GMO safety agency of the European Union (EFSA) discovered that both the CaMV promoter and the FMV promoter had wrongly been assumed by them (for almost 20 years) not to encode any proteins. In fact, the two promoters encode a large part of a small multifunctional viral protein that misdirects all normal gene expression and that also turns off a key plant defence against pathogens. EFSA tried to bury their discovery. Unfortunately for them, we spotted their findings in an obscure scientific journal. This revelation forced EFSA and other regulators to explain why they had overlooked the probability that consumers were eating an untested viral protein.
This list of significant scientific concerns about GMOs is by no means exhaustive. For example, there are novel GMOs coming on the market, such as those using double stranded RNAs (dsRNAs), that have the potential for even greater risks (Latham and Wilson 2015).

The True Purpose of GMOs

Science is not the only grounds on which GMOs should be judged. The commercial purpose of GMOs is not to feed the world or improve farming. Rather, they exist to gain intellectual property (i.e. patent rights) over seeds and plant breeding and to drive agriculture in directions that benefit agribusiness. This drive is occurring at the expense of farmers, consumers and the natural world. US Farmers, for example, have seen seed costs nearly quadruple and seed choices greatly narrow since the introduction of GMOs. The fight over GMOs is not of narrow importance. It affects us all.
Nevertheless, specific scientific concerns are crucial to the debate. I left science in large part because it seemed impossible to do research while also providing the unvarnished public scepticism that I believed the public, as ultimate funder and risk-taker of that science, was entitled to.
Criticism of science and technology remains very difficult. Even though many academics benefit from tenure and a large salary, the sceptical process in much of science is largely lacking. This is why risk assessment of GMOs has been short-circuited and public concerns about them are growing. Until the damaged scientific ethos is rectified, both scientists and the public are correct to doubt that GMOs should ever have been let out of any lab.
(An earlier version of this article appeared at http://nutritionstudies.org/)
(1) Two references on the anthrax issue (added Sept 2nd): Helgason, E., O. A. Økstad, D. A. Caugant, H. A. Johansen, A. Fouet, M. Mock, I. Hegna, and A.-B. Kolstø. 2000. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—one species on the basis of genetic evidence. Appl. Environ. Microbiol. 66: 2627-2630.
And:
Adelaida M. Gaviria Rivera, Per Einar Granum, Fergus G. Priest. 2000. Common occurrence of enterotoxin genes and enterotoxicity in Bacillus thuringiensis. FEMS Microbiology Letters 190 (2000) 151-155; http://dx.doi.org/10.1111/j.1574-6968.2000.tb09278.x

References

Bøhn, T, Cuhra, M, Traavik, T, Sanden, M, Fagan, J and Primicerio, R (2014) Compositional differences in soybeans on the market: Glyphosate accumulates in Roundup Ready GM soybeans. Food Chemistry 153: 207-215.
Droge W, Broer I, and Puhler A. (1992) Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide L-phosphinothricin (glufosinate) differently from untransformed plants. Planta 187: 142-151.
Lantz S et al., (2014) Glufosinate binds N-methyl-D-aspartate receptors and increases neuronal network activity in vitro. Neurotoxicology 45: 38-47.
Latham JR and Wilson AK (2015) Off -­ target Effects of Plant Transgenic RNAi: Three Mechanisms Lead to Distinct Toxicological and Environmental Hazards.
Mizuki, E, Et Al., (1999) Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell- killing action on human cancer cells. J. Appl. Microbiol. 86: 477–486.
Vachon V, Laprade R, Schwartz JL (2012) Current models of the mode of action of Bacillus thuringiensis insecticidal crystal proteins: a critical review. Journal of Invertebrate Pathology 111: 1–12.

GE Soybeans Give Altered Milk and Stunted Offspring, Researchers Find

October 26, 2015 

By Jonathan Latham, PhD
http://www.independentsciencenews.org/news/ge-soybeans-give-altered-milk-and-stunted-offspring-researchers-find/
Pregnant goats fed with genetically engineered (GE) soybeans have offspring who grow more slowly and are shorter, according to a new Italian study (Tudisco et al., 2015). Publishing in the journal of Small Ruminant Research, the researchers were testing the results of supplementing the feed of female goats with Roundup Ready GE soybeans.  Roundup Ready soybeans are engineered to resist the herbicide Roundup and are sold by agribusiness giant Monsanto. They are some of the most widely grown soybeans in the world.
The reduced growth of the goat kids was attributed by the researchers to their observation that the milk of the GE-fed mothers was significantly less nutritious and contained less of the IgG antibodies important for early growth.

Cilentana Goats, Italy

“This was a carefully conducted study” commented Dr Judy Carman, Director of the Institute of Health and Environmental Research, Australia. She was not involved in the research, but told Independent Science News that:
“The differences in the composition of the colostrum between the mothers fed the GE soy and the non-GE soy were particularly striking.  The colostrum from the GE-fed mothers contained only 2/3 of the fat, 1/3 of the protein and close to half of the IgG of the mothers fed the non-GM soy.”
To carry out these experiments the researchers divided pregnant female Cilentana goats into four groups, sixty days before kidding. Two of the groups were fed goat food containing GE Roundup Ready soybeans (at two different concentrations). The other two groups were fed conventional (non-GE) soybeans, also at two different concentrations.
After the mothers gave birth all offspring were fed only with their mother’s milk for sixty days. The growth of these kids was measured twice. After both thirty days and sixty days the kids of GE-fed mothers were approximately 20% lower in weight and shorter in stature. Both these differences were statistically significant.
Lower offspring weights were not the only unexpected findings. The researchers also found that the milk of GE-fed goats was lower in protein and fat. This difference in milk quality was large (6% protein in both GE-fed groups versus 18% in both non-GE fed groups) for the first few weeks after birth but gradually disappeared—even though the mothers continued to be fed the GE soybeans. Additionally, the researchers also found that the colostrum produced by GE-fed mothers had low amounts of IgG antibodies. These antibodies are important for growth and for healthy immune development.
A third difference noted by the researchers was that transgenic DNA could be detected in the colostrum of most (10/16) of the GE-fed goats. No transgene DNA was detected in the milk of goats fed non-GE soybeans. This is not the first time that transgene DNA (or non-transgenic DNA) has been found in the milk of ruminants, however.
Interestingly, the researchers found that all of the kids were of similar size at birth, regardless of whether their mothers ate Roundup Ready GE soybeans or not. The researchers therefore proposed that the stunting of the offspring of GE-fed mothers reflected a milk deficiency. Presumably either the lower nutritional value of the colostrum and milk of GE-fed mothers or the colostrum antibody differences that were observed. The authors noted that low IgG antibody levels in colostrum are correlated in other ruminants with slower growth and also that IgG antibodies are known to have a role in nutrient absorption because they promote gut development in newborns.
The researchers did not discuss whether the transgene DNA fragments found in the milk played a role in altering kid development.
This result is the strongest demonstration so far of altered growth and development in offspring of GE-fed mothers. The same researchers in 2010 showed altered activity of the lactic dehydrogenase enzyme in kids fed milk from mothers that ate GE Roundup Ready soybeans. In that previous study however, no additional effects on goat offspring were detected (Tudisco et al., 2010).
“It is already known that Roundup Ready soybeans have various defects including a Manganese deficiency. Yet regulators and GMO developers have continuously dismissed credible reports of GMO crops causing apparent harm to animals, from many different research groups.” Said Dr Allison Wilson of The Bioscience Resource Project. “Hopefully they will not ignore yet another study.”
References
Tudisco R., V. Mastellone, M. I. Cutrignelli, P. Lombardi, F. Bovera, N. Mirabella, G. Piccolo, S. Calabrò, L. Avallone and F. Infascelli (2010) Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings. Animal 4: 1662-1671.
Tudisco R., S. Calabrò, M.I. Cutrignelli, G. Moniello, M. Grossi, V. Mastellone, P. Lombardi, M.E. Peroa, F. Infascelli (2015) Genetically modified soybean in a goat diet: Influence on kid performance. Small Ruminant Research 126: 67–74.